• Volume 12, 2022
  • Volume 11, 2021
  • Volume 10, 2020
  • Volume 9, 2019
  • Volume 8, 2018
  • Volume 7, 2017
  • Volume 6, 2016
  • Volume 5, 2015
  • Volume 4, 2014
  • Volume 3, 2013
  • Volume 2, 2012
  • Volume 1, 2011
Image Credit: BioProtoc.4557

Current Issue in 2022

Volume: 12, Issue: 22

Biological Engineering

A Fast and Efficient Decellularization Method for Tissue Slices Authors:  Maria Narciso, Anna Ulldemolins, Constança Júnior, Jorge Otero, Daniel Navajas, Ramon Farré, Núria Gavara and Isaac Almendros, date: 11/20/2022, view: 646, Q&A: 0

The study and use of decellularized extracellular matrix (dECM) in tissue engineering, regenerative medicine, and pathophysiology have become more prevalent in recent years. To obtain dECM, numerous decellularization procedures have been developed for the entire organ or tissue blocks, employing either perfusion of decellularizing agents through

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Developmental Biology

Monitoring the Recruitment and Fusion of Autophagosomes to Phagosomes During the Clearance of Apoptotic Cells in the Nematode Caenorhabditis elegans Authors:  Omar Peña-Ramos and Zheng Zhou, date: 11/20/2022, view: 526, Q&A: 0

During an animal's development, a large number of cells undergo apoptosis, a suicidal form of death. These cells are promptly phagocytosed by other cells and degraded inside phagosomes. The recognition, engulfment, and degradation of apoptotic cells is an evolutionarily conserved process occurring in all metazoans. Recently, we discovered a novel

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Drug Discovery

In vitro Assay to Evaluate Cation Transport of Ionophores Authors:  Huriye D. Uzun, Melissa Vázquez-Hernández, Julia E. Bandow and Thomas Günther Pomorski, date: 11/20/2022, view: 498, Q&A: 0

Ion homeostasis is a fundamental regulator of cellular processes and depends upon lipid membranes, which function as ion permeability barriers. Ionophores facilitate ion transport across cell membranes and offer a way to manipulate cellular ion composition. Here, we describe a calcein quenching assay based on large unilamellar vesicles that we

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Microbiology

Fluorescent Binding Protein Sensors for Detection and Quantification of Biochemicals, Metabolites, and Natural Products Authors:  Salete M. Newton and Phillip E. Klebba, date: 11/20/2022, view: 805, Q&A: 0

Membrane transporters and soluble binding proteins recognize particular nutrients, metabolites, vitamins, or ligands. By modifying genetically engineered single cysteine residues near the active sites of such proteins with extrinsic maleimide fluorophores, the approaches that we report create sensitive fluorescent sensors that detect, quantify,

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Babesia duncani in Culture and in Mouse (ICIM) Model for the Advancement of Babesia Biology, Pathogenesis and Therapy Authors:  Vandana Kumari, Anasuya C. Pal, Pallavi Singh and Choukri Ben Mamoun, date: 11/20/2022, view: 314, Q&A: 0

Babesiosis is a tick-borne disease caused by pathogens belonging to the genus Babesia. In humans, the disease presents as a malaria-like illness and can be fatal in immunocompromised and elderly people. In the past few years, human babesiosis has been a rising concern worldwide. The disease is transmitted through tick bite, blood transfusion, and

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Hypochlorite Stress Assay for Phenotypic Analysis of the Halophilic Archaeon Haloferax volcanii Using an Improved Incubation Method and Growth Monitoring Authors:  Paula Mondragon, Sungmin Hwang, Amy Schmid and Julie A. Maupin-Furlow, date: 11/20/2022, view: 317, Q&A: 0

The study of haloarchaea provides an opportunity to expand understanding of the mechanisms used by extremophiles to thrive in and respond to harsh environments, including hypersaline and oxidative stress conditions. A common strategy used to investigate molecular mechanisms of stress response involves the deletion and/or site-directed mutagenesis

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Neuroscience

Fluorescence Screens for Identifying Central Nervous System–Acting Drug–Biosensor Pairs for Subcellular and Supracellular Pharmacokinetics Authors:  Zoe G. Beatty, Anand K. Muthusamy, Elizabeth K. Unger, Dennis A. Dougherty, Lin Tian, Loren L. Looger, Amol V. Shivange, Kallol Bera, Henry A. Lester and Aaron L. Nichols, date: 11/20/2022, view: 587, Q&A: 0

Subcellular pharmacokinetic measurements have informed the study of central nervous system (CNS)–acting drug mechanisms. Recent investigations have been enhanced by the use of genetically encoded fluorescent biosensors for drugs of interest at the plasma membrane and in organelles. We describe screening and validation protocols for identifying hit

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In vitro Preparation of Homogenous Actin Filaments for Dynamic and Electrophoretic Light Scattering Measurements Authors:  Ernesto Alva, Annitta George, Lorenzo Brancaleon and Marcelo Marucho, date: 11/20/2022, view: 350, Q&A: 0

Actin filaments are essential for various biological activities in eukaryotic cellular processes. Available in vitro experimental data on these systems often lack details and information on sample preparation protocols and experimental techniques, leading to unreproducible results. Additionally, different experimental techniques and polymerization

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Stem Cell

Using BODIPY FL-Sphingolipid Analogs to Study Sphingolipid Metabolism in Mouse Embryonic Stem Cells Authors:  Wei Fan and Xiaoling Li, date: 11/20/2022, view: 347, Q&A: 0

Sphingolipids are important structural components of cellular membranes. They also function as prominent signaling molecules to control a variety of cellular events, such as cell growth, differentiation, and apoptosis. Impaired sphingolipid metabolism, particularly defects in sphingolipid degradation, has been associated with many human diseases.

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Systems Biology

Proteome Integral Solubility Alteration (PISA) Assay in Mammalian Cells for Deep, High-Confidence, and High-Throughput Target Deconvolution Authors:  Xuepei Zhang, Olga Lytovchenko, Susanna L. Lundström, Roman A. Zubarev and Massimiliano Gaetani, date: 11/20/2022, view: 511, Q&A: 0

Chemical proteomics focuses on the drug–target–phenotype relationship for target deconvolution and elucidation of the mechanism of action—key and bottleneck in drug development and repurposing. Majorly due to the limits of using chemically modified ligands in affinity-based methods, new, unbiased, proteome-wide, and MS-based

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Efficient Generation of Genome-wide Libraries for Protein–ligand Screens Using Gibson Assembly Authors:  Tamara Sternlieb, Mira Loock, Mengjin Gao and Igor Cestari, date: 11/20/2022, view: 488, Q&A: 0

Genome-wide screens using yeast or phage displays are powerful tools for identifying protein–ligand interactions, including drug or vaccine targets, ligand receptors, or protein–protein interactions. However, assembling libraries for genome-wide screens can be challenging and often requires unbiased cloning of 105–107 DNA

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