• Volume 12, 2022
  • Volume 11, 2021
  • Volume 10, 2020
  • Volume 9, 2019
  • Volume 8, 2018
  • Volume 7, 2017
  • Volume 6, 2016
  • Volume 5, 2015
  • Volume 4, 2014
  • Volume 3, 2013
  • Volume 2, 2012
  • Volume 1, 2011
Image Credit: BioProtoc.4508

Biochemistry

Protein Tyrosine Phosphatase Biochemical Inhibition Assays Authors:  Marek R. Baranowski, Jiaqian Wu, Ye Na Han, Lester J. Lambert, Nicholas D. P. Cosford and Lutz Tautz, date: 09/20/2022, view: 909, Q&A: 0

Disturbance of the dynamic balance between protein tyrosine phosphorylation and dephosphorylation, modulated by protein tyrosine kinases (PTKs) and protein tyrosine phosphatases (PTPs), is known to be crucial for the development of many human diseases. The discovery of agents that restore this balance has been the subject of many drug

...
More >>
In vitro Fluorescence Imaging–based Actin Bundling Assay Authors:  Anjelika Gasilina and Paul A. Randazzo, date: 09/20/2022, view: 1016, Q&A: 0

Understanding the molecular and structural mechanisms that govern the assembly and organization of higher-order actin architecture requires the use of in vitro actin binding and bundling assays. Crosslinking of actin filaments into bundles can be monitored in vitro via several techniques, including negative staining/electron microscopy, low-speed

...
More >>

Biophysics

Serial Room Temperature Crystal Loading, Data Collection, and Data Processing of Human Glutaminase C in Complex with Allosteric Inhibitors Authors:  Shawn K. Milano, D. Marian Szebenyi and Richard A. Cerione, date: 09/20/2022, view: 508, Q&A: 0

Cancer cells often overexpress glutaminase enzymes, in particular glutaminase C (GAC). GAC resides in the mitochondria and catalyzes the hydrolysis of glutamine to glutamate. High levels of GAC have been observed in aggressive cancers and the inhibition of its enzymatic activity has been shown to reduce their growth and survival. Numerous GAC

...
More >>
A Novel Imaging Technique for The On-site Assessment of Renal Biopsy Specimens Authors:  Tomoaki Takata, Hajime Isomoto, Takuji Iyama and Kentaro Yamada, date: 09/20/2022, view: 527, Q&A: 0

When performing renal biopsy, it is necessary to identify the cortex, where glomeruli are exclusively distributed, to ensure the quality of the specimen for histological diagnosis. However, conventional methods using a stereomicroscope or magnifying lens often fail to clarify the quality of the specimen. We have established a fluorescent-based

...
More >>

Cell Biology

Human Skin Explant Preparation and Culture Authors:  Jessica L. Shannon, Stephen J. Kirchner and Jennifer Y. Zhang, date: 09/20/2022, view: 731, Q&A: 0

The ex vivo experimentation with surgically discarded human skin represents a unique methodology amenable for mechanism and pharmacologic agent studies without the involvement of human subjects. Here, we describe a protocol that includes preparation, culture, and stimulation of human skin explants, and subsequent analyses by quantitative reverse

...
More >>

Computational Biology and Bioinformatics

Tissue-Specific, Genome-wide Mapping of R-loops in Drosophila Using MapR Authors:  Juan Jauregui-Lozano, Kendall Cottingham and Hana Hall, date: 09/20/2022, view: 697, Q&A: 0

R-loops, or RNA:DNA hybrids, are structures that arise co-transcriptionally when a nascent RNA hybridizes back with the template ssDNA, leading to a displaced ssDNA. Because accumulation of R-loops can lead to genomic instability and loss of cellular homeostasis, it is important to determine the genome-wide distribution of R-loops in

...
More >>

Microbiology

Analysis of Lipid-linked Oligosaccharides Synthesized in vivo in Schizosaccharomyces pombe Authors:  Ayelen Valko, Giovanna L. Gallo, Ariel D. Weisz, Armando J. Parodi and Cecilia D’Alessio, date: 09/20/2022, view: 588, Q&A: 0

Dolichol diphosphate-linked oligosaccharides (LLO) are the sugar donors in N-glycosylation, a fundamental protein post-translational modification of the eukaryotic secretory pathway. Defects in LLO biosynthesis produce human Congenital Disorders of Glycosylation Type I. The synthesis of LLOs and the transfer reactions to their protein

...
More >>

Neuroscience

In situ Dephosphorylation Assay with Recombinant Nil Phosphatase Authors:  Nilay Nandi, Charles Tracy and Helmut Krämer, date: 09/20/2022, view: 944, Q&A: 0

The activity of numerous autophagy-related proteins depends on their phosphorylation status, which places importance on understanding the responsible kinases and phosphatases. Great progress has been made in identifying kinases regulating autophagy, but much less is known about the phosphatases counteracting their function. Genetic screens and

...
More >>
Characterizing Hedonic Responses to Flavors Paired with Internal Pain and Nausea through the Taste Reactivity Test in Rats Authors:  Matías López, Dominic M. Dwyer, Patricia Gasalla, Claudia Jove and Azucena Begega, date: 09/20/2022, view: 823, Q&A: 0

Feeding behavior is a complex experience that involves not only sensory (i.e., visual, odor, taste, or texture) but also affective or emotional aspects (i.e., pleasure, palatability, or hedonic value) of foods. As such, behavioral tests that assess the hedonic impact of foods are necessary to fully understand the factors involved in ingestive

...
More >>

Plant Science

14C-paraquat Efflux Assay in Arabidopsis Mesophyll Protoplasts Authors:  Jin-Qiu Xia, Qian-Qian Liu and Cheng-Bin Xiang, date: 09/20/2022, view: 589, Q&A: 0

Weeds compete with crops for growth resources, causing tremendous yield losses. Paraquat is one of the three most common non-selective herbicides. To study the mechanisms of paraquat resistance, we need to trace the movement of paraquat in plants and within the cell. 14C is a radioactive carbon isotope widely used to trace substances of

...
More >>
We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.