Developmental Biology

Categories

    Protocols in Current Issue
    Monitoring the Recruitment and Fusion of Autophagosomes to Phagosomes During the Clearance of Apoptotic Cells in the Nematode Caenorhabditis elegans
    Authors:  Omar Peña-Ramos and Zheng Zhou, date: 11/20/2022, view: 526, Q&A: 0

    During an animal's development, a large number of cells undergo apoptosis, a suicidal form of death. These cells are promptly phagocytosed by other cells and degraded inside phagosomes. The recognition, engulfment, and degradation of apoptotic cells

    ...
    Monitoring the Recruitment and Fusion of Autophagosomes to Phagosomes During the Clearance of Apoptotic Cells in the Nematode Caenorhabditis elegans
    Authors:  Omar Peña-Ramos and Zheng Zhou, date: 11/20/2022, view: 526, Q&A: 0
    [Abstract]

    During an animal's development, a large number of cells undergo apoptosis, a suicidal form of death. These cells are promptly phagocytosed by other cells and degraded inside phagosomes. The recognition, engulfment, and degradation of apoptotic cells is an evolutionarily conserved process occurring in all metazoans. Recently, we discovered a novel

    ...
    MiniSOG2-mediated Specific Photoablation of Motor Neurons in Ascidian Embryos
    Authors:  Madoka K. Utsumi, Taichi Akahoshi, Kotaro Oka and Kohji Hotta, date: 10/20/2022, view: 749, Q&A: 0
    [Abstract]

    When understanding the neuronal function of a specific neural circuit, single-cell level photoablation of a targeted cell is one of the useful experimental approaches. This protocol describes a method to photoablate specific motor neurons via the mini singlet oxygen generator (miniSOG2), a light–oxygen–voltage (LOV)-based optogenetic

    ...
    First-line Evaluation of Sperm Parameters in Mice (Mus musculus)
    Author:  Guillaume Martinez, date: 10/20/2022, view: 581, Q&A: 0
    [Abstract]

    Infertility has become a major public health problem, with a male factor involved in about half the cases. Mice are the most widely used animal model in reproductive biology research laboratories, but changes in sperm parameters in mice can be subtle and, in the absence of official guidelines, it is important that analyses are carried out in a

    ...
    In situ Dephosphorylation Assay with Recombinant Nil Phosphatase
    Authors:  Nilay Nandi, Charles Tracy and Helmut Krämer, date: 09/20/2022, view: 944, Q&A: 0
    [Abstract]

    The activity of numerous autophagy-related proteins depends on their phosphorylation status, which places importance on understanding the responsible kinases and phosphatases. Great progress has been made in identifying kinases regulating autophagy, but much less is known about the phosphatases counteracting their function. Genetic screens and

    ...
    Generation of iMyoblasts from Human Induced Pluripotent Stem Cells
    [Abstract]

    Skeletal muscle stem cells differentiated from human-induced pluripotent stem cells (hiPSCs) serve as a uniquely promising model system for investigating human myogenesis and disease pathogenesis, and for the development of gene editing and regenerative stem cell therapies. Here, we present an effective and reproducible transgene-free protocol for

    ...
    Co-differentiation and Co-maturation of Human Cardio-pulmonary Progenitors and Micro-Tissues from Human Induced Pluripotent Stem Cells
    Authors:  Wai Hoe Ng, Barbie Varghese and Xi Ren, date: 08/20/2022, view: 1469, Q&A: 0
    [Abstract]

    Currently, there are several in vitro protocols that focus on directing human induced pluripotent stem cell (hiPSC) differentiation into either the cardiac or pulmonary lineage. However, these systemsprotocols are unable to recapitulate the critical exchange of signals and cells between the heart and lungs during early development. To address

    ...
    Preparation of a Single-cell Suspension from Drosophila Wing Imaginal Discs
    Authors:  Shu Yang, Brooke Sears and Xiaoyan Zheng, date: 08/20/2022, view: 1058, Q&A: 0
    [Abstract]

    The wing imaginal discs in Drosophila larvae are a pair of sac-like structures that later form the wings of the adult fly. During the past decades, wing discs have been used as a simple and accessible model system, for identifying genes and deciphering signaling cascades that play crucial roles in many aspects of development. In this protocol, we

    ...
    Fluorescence Imaging of 3D Cell Models with Subcellular Resolution
    [Abstract]

    Over the past years, research has made impressive breakthroughs towards the development and implementation of 3D cell models for a wide range of applications, such as drug development and testing, organogenesis, cancer biology, and personalized medicine. Opposed to 2D cell monolayer culture systems, advanced 3D cell models better represent the in

    ...
    Analysis of Caenorhabditis elegans Aging-related Neurodegeneration in Chemosensory Neurons
    Authors:  Cira Crespo and Roberto Grau, date: 07/20/2022, view: 1073, Q&A: 0
    [Abstract]

    Aging and neuronal deterioration constitute important risk factors for the development of neuronal-related diseases, such as different dementia. The nematode Caenorhabditis elegans has emerged as a popular model system for studying neurodegeneration diseases, due to its complete neuronal connectivity map. DiI is a red fluorescent dye that can

    ...
    In vivo Characterization of Endogenous Protein Interactomes in Drosophila Larval Brain, Using a CRISPR/Cas9-based Strategy and BioID-based Proximity Labeling
    Authors:  Ezgi Uçkun, Georg Wolfstetter, Johannes Fuchs and Ruth H. Palmer, date: 07/05/2022, view: 1667, Q&A: 0
    [Abstract]

    Understanding protein-protein interactions (PPIs) and interactome networks is essential to reveal molecular mechanisms mediating various cellular processes. The most common method to study PPIs in vivo is affinity purification combined with mass spectrometry (AP–MS). Although AP–MS is a powerful method, loss of weak and transient

    ...



    We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.